human hepatic normal cell line hl7702  (Procell Inc)

Journal: Cell Death Discovery

Article Title: pir-hsa-216911 inhibit pyroptosis in hepatocellular carcinoma by suppressing TLR4 initiated GSDMD activation

doi: 10.1038/s41420-024-02285-9

Figure Lengend Snippet: A The diagram illustrates our small RNA sequencing pipeline. B The heatmap displays the quality of reads based on their length. The read quality mainly concentrated at 38, ranging from 1 to 45 nt. C This histogram provides a visual representation of the distribution of sRNA tag length. Aside from the primary peak indicating miRNA (19–25), the secondary peak corresponds to piRNA (24–30). D The donut plot and list show sRNA tag mapping and annotation results. piRNAs represented only 1–3% of sequenced sRNAs. E Volcano plots show the distribution of differentially expressed genes in the three HCC cell lines compared to normal liver cells. F The Venn diagram shows the DEG counts in each HCC cell line. G The Venn diagram shows the DEG counts in each set. H Histogram display reads count distribution in all cell lines in sRNA sequencing result. I qPCR showed decreased pir-hsa-216911 expression in HCC cell lines compared to normal liver cells; ***p < 0.001, ** p < 0.01 by student-T tests against piRNA expression levels in HL7702.

Article Snippet: Human normal hepatic cell line HL7702 and hepatocellular carcinoma cell lines Huh7, HepG2, and Hep3B were obtained from the China Center for Type Culture Collection.

Techniques: RNA Sequencing, Sequencing, Expressing